Development of an optical microscope for 3D cellular imaging in microfluidics chip
Published : 11 January 2019
Though still confronted with multiple challenges, regenerative medicine (tissue reconstruction) offers many promises for the future. One of the current limitations remains the tissue vascularization. Ex vivo, this development requires the use of microfluidic systems to mimic vasculogenesis. A challenge remains however to be addressed: the characterization of the reconstructed tissues without altering their functionality to allow implantation. The aim of the project is to develop an optical imaging system allowing a morphological and functional 3D characterization on a micrometric scale of vascularized tissues inside a fluidic chamber. The successful candidate will be responsible for developing and characterizing an innovative optical microscopy system that meets the expected performance to characterize the samples, including spatial resolution, field size, and sensitivity. Coupling and integration with microfluidics systems will be part of the objectives of this development. The candidate will work in a multidisciplinary environment and will have to interact strongly with the biologists and micro-fluidicists of the project to carry out this work.