biophysical analysis of exosomes for diagnosis, and precision medicine
Published : 8 February 2020
Cancer is the second leading cause of mortality in Europe, accounting for ~1.3M deaths in 2015. In this thesis we propose a new scenario for cancer liquid biopsy, based on the biophysical fingerprints of exosomes. Recent studies have shown exosomes to mediate signals for hypoxia driven epithelial-mesenchymal transition and metastasis, suggesting they could be powerful cancer biomarkers directly accessible in bodily fluids. There is increasing evidence of the specific biophysical link between exosomes and the parent cell. This thesis aims at developing and implementing new nanomechanical and microfluidics methods to investigate the biophysical signatures of exosomes from different cell lines, including healthy controls and cancer cells, in a perspective of early-cancer diagnosis, and precision medicine.