Development and characterization of innovative biosensors on optical fibers: application to the detection of cells/bacteria
Published : 26 March 2019
Optical fibres are a minimally invasive device commonly used in medicine to image tissues in vivo by endoscopy. For in vitro analysis, biochips are widely used for multiparametric detection of various biological compounds. The objective of the thesis is to couple these two technologies in order to develop a new tool able of performing remote, real time, in situ, unlabeled and multiplexed molecular and/or cellular analysis. Applied to the detection of cells in the broad sense (cells, bacteria, yeasts, fungi), this new tool could be used in various applications: analysis of a drop of blood at home, in vivo diagnosis via the identification of pathogens inside the human body, prevention of nosocomial diseases through monitoring the development of bacteria in medical devices involving narrow tubes (drain, catheter, urinary probe…), quality control (detection of bacteria in equipment difficult to access).
The proposed approach is based on the use of a microstructured optical fibre network (multi-fibre assembly) functionalized with biological probes on one end. Detection methods will be based on optical phenomena (interferometry, surface plasmon resonance, autofluorescence). The doctoral student will participate in the modelling, development and characterization of biosensors. He/she will then be involved in the implementation of biosensors for application to the detection of bacteria or cells.