Study of a panel of exhaled biomarkers in humans, for application to respiratory exposure biomonitoring to nanoparticles
Published : 26 March 2019
The use of manufactured nanoparticles (NPs) is steadily increasing in the international research and industrial sectors, while their toxicity is not yet clearly understood. Ultrafine particulate pollution is also largely implicated in the respiratory and cardiovascular effects of populations. Atmospheric measurements are essential for assessing exposure but need to be supplemented by a biological approach, the only one allowing the evaluation of the internal dose actually absorbed by the organism and potential biological effects. At the respiratory level, exhaled breath condensate has the dual benefit of being representative of inhalation exposure and of being collected in a simple and non-invasive manner. The proposed project aims to develop an innovative method for simultaneous analysis of a panel of biomarkers (oxidative stress, inflammation) in exhaled air, using high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS / MS). The final method will be tested on volunteers and groups of subjects of interest, in order to assess its adequacy for the monitoring of exposure to nanoparticles in humans.